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KMID : 0603820170230030223
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Journal of Experimental & Biomedical Science
2017 Volume.23 No. 3 p.223 ~ p.229
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Genetic Variations of Aspergillus fumigatus Clinical Isolates from Korea
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Kim Sung-Hyun
Ma Pan-Gon
Park Young-Seok
Yu Young-Bin
Hwang Kyu-Jam
Kim Young-Kwon
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Abstract
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Fungal infections by human pathogenic fungi are increasing globally in elderly, children and immune suppressed or deficient patients. Aspergillus fumigatus is one of the well-known pathogenic fungi and causes aspergilloses in human world widely. However, current identification and classification methods based on its phenotypic characteristics still have limitations. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to analyze genetic variations of A. fumigatus clinical isolates, a total of six housekeeping genes were amplified by PCR using specific primer pairs and multi-locus sequence typing (MLST) assay. Results from phylogenetic tree analysis showed that most A. fumigatus strains (88.9%) from respiratory specimens were classified into cluster A and B, and approximately half of A. fumigatus strains (46%) from non-respiratory specimens were classified into cluster C and D. Although the sample size was limited, genetic characteristics of A. fumigatus clinical isolates according to their origins were very similar and well-correlated with other clinical data.
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KEYWORD
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Aspergillus fumigatus , Clinical isolates , Genetic variations , Multi-locus sequence typing , Genotypes
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